OR16-4 Genomic Interrogation of the Phytoestrogen Genistein in Ovarian Cancer Cells

Abstract Although studies have shown beneficial effects of the phytoestrogen genistein on reducing ovarian cancer risk, little is known at molecular level regarding how inhibits growth, with estrogen receptor beta (ERb), a hormone-regulated transcription factor, receiving bulk attention. When endogenous and exogenous ligands engage ERb, binds to regulatory regions in genome as enhancers, where it associates coregulatory proteins, increases histone modifications associated active promotes chromatin looping, stimulates enhancer production RNAs, ultimately regulates target gene transcription. Since signaling pathways converge transcriptome level, an unbiased approach needed examine acts suppress growth beyond ERb. We previously used global run-on sequencing (GRO-seq), high-throughput method that identifies location orientation all transcriptionally RNA polymerases across genome, identify enhancers based enrichment aforementioned genomic features. To determine responses upon treatment cells, we PRO-seq characterize landscape OVCAR3 OVCAR4 cell lines treated genistein. The advantage this lack priori assumptions which are regulated by genistein, since allows pathway determination universe enhancers. Ultimately, will allow us uncover ERb ligand change cells. Presentation: Sunday, June 12, 2022 11:45 a.m. - 12:00 p.m.